人工感染三角帆蚌病毒性疾病的组织病理学观察(英文)

钟蕾; 肖调义; 黄倢; 戴良英; 刘晓燕

doi:10.3724/SP.J.1035.2011.006661

人工感染三角帆蚌病毒性疾病的组织病理学观察(英文)

湖南农业大学, 长沙 410128
2. 中国水产科学院黄海水产研究所, 海洋渔业可持续发展重点实验室, 青岛 266071

基金项目:

The Science and Technology Support Projects of China(Grant No.2007BAD87B11)

the Science and Technology Research Projects,Hunan Province,Grant No.06NK3049)

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出版历程
- 收稿日期: 2010-07-20
- 修回日期: 2011-03-11
- 发布日期: 2011-07-24

HISTOPATHOLOGICAL EXAMINATION OF BIVALVE MUSSEL HYRIOPSIS CUMINGII LEA ARTIFICIALLY INFECTED BY VIRUS

College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Yellow Sea Fisheries Research Institute, Chinese Ministry of Agriculture, Qingdao 266071, China

摘要

摘要: 从自然发病的病蚌中提取出病毒,经人工感染健康三角帆蚌后呈现出典型的病理变化。组织学观察显示消化腺、胃、肠、外套膜、斧足和鳃等均出现大量细胞空泡化、部分细胞肿大、上皮细胞排列不紧密,结缔组织不完整,部分细胞溶解甚至脱落等,表明它们均为病毒损害的主要靶器官。透射电镜观察显示大量砂样病毒存在于患病三角帆蚌不同器官中。病毒粒子为直径约120 nm的圆球形,表面具有明显的纤突,无囊膜结构。被感染细胞呈现明显的病理变化,包括细胞质大片空泡化,细胞器相对减少或缺失,细胞核电子密度加深,肠黏膜上皮细胞不同程度的变性和坏死,消化腺细胞核变形肿大,鳃小片上皮细胞坏死脱落,颗粒细胞的核膜溶解等。
- 三角帆蚌 /
- 砂样病毒 /
- 显微结构 /
- 超微结构 /
- 组织病理学
Abstract: The pathogen Hyriopsis cumingii Plague Virus isolated from the diseased H. cumingii Lea has been artificiallytransferred to the healthy H. cumingii Lea. The infected H. cumingii Lea has displayed some typical pathologicalchanges. Observations by histopathology have showed that most of organs including the digestive gland, stomach andintestine are the primary targets of the plague virus. Lesions have been observed, accompanied with a number of emptyareas, cell vacuolization and some cells falling-off. Arenavirus-like particles have been detected by using transmissionelectron microscopic (TEM) examination on investigated organs of the artificially infected bivalve mussel Hyriopsiscumingii. Virions are spherical with a size of 120 nm in diameter, and the outer layer of the intact arenavirus-like particleshave small protuberance and moderate electron-dense nucleicapsid. Under electronic microscope, the most obviouscellular pathologic feature is the large vacuole areas of cytoplasm, and there is the reduction or devoid of organelles inthe cytoplasm. Varying degrees of denaturalization and necrosis have been found in the intestinal mucosa epithelial cells.We have also observed that in cytopathic cells of digestive gland, the nuclei has been discovered to be amorphous andhypertrophied. There are denaturalizationand necrosis in the epithelial cell of gill and the nucleus membrane of granulocyteshave been dissolved.
- Hyriopsis cumingii Lea /
- Arenavirus-like /
- Microstructure /
- Ultrastructure /
- Histopathology

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