饲料中铜、钙水平对大黄鱼幼鱼生长、抗氧化酶及脂代谢酶活性的影响
EFFECTS OF DIETARY COPPER AND CALCIUM LEVELS ON GROWTH, ANTIOXIDANT ENZYME AND LIPID METABOLISM ENZYME ACTIVITIES OF JUVENILE LARGER YELLOW CROAKER LARIMICHTHYS CROCEA
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摘要: 为研究钙对摄食高铜饲料的大黄鱼幼鱼生长性能、抗氧化酶类及脂代谢酶类活性的影响,研究采用22双因子实验设计,配制了4组实验饲料,共包含2个铜水平(4和24 mg/kg)和2个钙水平(0.5%和3%),分别命名为: -Cu-Ca、+Cu-Ca、-Cu+Ca和+Cu+Ca,饲喂大黄鱼幼鱼[(4.050.31) g]10周,每个处理组设置3个重复。结果表明,高铜组(+Cu-Ca和+Cu+Ca)实验鱼的存活率、终末体质量和增重率显著低于低铜组(-Cu-Ca和-Cu+Ca)(P0.05); 高铜组实验鱼肝脏和肌肉中的铜含量显著高于低铜组,而高钙组(-Cu+Ca和+Cu+Ca)实验鱼肌肉中的铜含量却显著低于低钙组(-Cu-Ca和+Cu-Ca)(P0.05); 高铜组实验鱼肝脏中的糖原含量显著低于低铜组(P0.05); 实验鱼肝脏中的超氧化物歧化酶、过氧化物酶、谷胱甘肽过氧化物酶、6-磷酸葡糖酸脱氢酶、异柠檬酸脱氢酶和脂肪酸合成酶活性,高铜组显著低于低铜组,而高钙组却显著高于低钙组,但硫代巴比妥酸反应物含量和脂蛋白脂肪酶活性正好相反(P0.05); 饲料中铜和钙的交互作用显著影响了实验鱼肌肉中的铜含量、硫代巴比妥酸反应物含量、6-磷酸葡糖酸脱氢酶、脂肪酸合成酶和脂蛋白脂肪酶活性(P0.05)。研究表明,钙能够缓解摄食高铜饲料对大黄鱼幼鱼造成的伤害。Abstract: To evaluate effects of dietary copper and calcium on juvenile larger yellow croaker Larimichthys crocea, four experimental diets (-Cu-Ca, +Cu-Ca, -Cu+Ca and +Cu+Ca) were formulated containing two different levels of copper (4 and 24 mg/kg) and of cadmium (0.5% and 3%) to feed juvenile large yellow croaker (4.050.31) g in tripli-cate for 10 weeks. The results indicated that the addition of Cu decreased fish survival rate, final body weight and weight gain (P0.05). As expected, the Cu diets enhanced liver and muscle Cu concentrations, Interestingly, the Ca diets diminished muscle Cu concentration (P0.05). The supplement of Cu decreased liver glycogen concentration, liver superoxide dismutase, catalase, glutathione peroxidase, 6-phosphogluconate dehydrogenase, isocitrate dehydrogenase, and fatty acid synthase activities, but it it enhanced thiobarbituric acid reactive substance content and lipoprotein lipase activity (P0.05). The interaction between Cu and Ca on muscle Cu concentration, thiobarbituric acid reac-tive substance content, 6-phosphogluconate dehydrogenase, fatty acid synthase and lipoprotein lipase activities was identified (P0.05). This study indicated that calcium supplement mitigated injuries in fish by high copper intake.
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Keywords:
- Larger yellow croaker /
- Copper /
- Calcium /
- Antioxidant enzyme /
- Lipid metabolism enzyme
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