洪湖碘泡虫的再描述及其近缘种的鉴别性研究
REDESCRIPTION OF MYXOBOLUS HONGHUENSIS LIU ET AL. 2012 AND IDENTIFICATION ON ITS GENETIC RELATED SPECIES
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摘要: 研究基于形态特征和18S rDNA序列相似度、遗传距离、变异位点、GC含量和系统发育比较分析,对采自河南龙湖的寄生于异育银鲫鳃部的一种黏孢子虫以及相似性极高且易混淆的黏孢子虫种类(洪湖碘泡 Myxobolus honghuensis Liu,et al. 2012、瓶囊碘泡虫Myxobolus ampullicapsulatus Zhao,et al. 2008、咽碘泡虫Myxobolus pharynae Lu,et al. 2012和吴李碘泡虫Myxobolus wulii (Wu Li,1986) 进行了系统的鉴别研究。研究结果显示: 河南龙湖异育银鲫鳃部所检获的黏孢子虫为洪湖碘泡虫,该种群对所寄生的异育银鲫未造成疾病症状; 咽碘泡虫与洪湖碘泡虫各种群在形态上极相似,两者间18S rDNA序列相似度为99%-100%,遗传距离为0-0.0013,GC含量均为44.31%,变异位点为2个,表明咽碘泡虫与洪湖碘泡虫应为同一物种。Abstract: The present study investigated the relationship of Myxobolus species from gills of Carassius auratus gibelio, Long Lake, Henan with other similar species such as Myxobolus honghuensis, Myxobolus ampullicapsulatus, Myxobo-lus pharynae, and Myxobolus wulii based on the comparative analysis of morphological characters, sequence similarity, genetic distance, mutation sites, GC content of 18S rDNA, and the phylogenic trees. The results indicated that the gill parasitic Myxobolus species from Carassius auratus gibelio, Long Lake, Henan was Myxobolus honghuensis, and no obvious host symptom was observed by this species. The further investigation showed that Myxobolus pharynae and Myxobolus honghuensis were similar in morphology and in sequence that reached 99%-100%, and that the genetic distance was only 0-0.0013, and that they had the same 44.31%, GC content with only two mutation sites. These re-sults indicated that these two species should the same species.
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Keywords:
- Myxobolus honghuensis /
- Myxobolus pharynae /
- Morphology /
- 18S rDNA
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