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摘要: 研究以自由生长的爪哇伪枝藻(Free-living S. javanicum, fs)和分离自地衣的爪哇伪枝藻(Symbiotic S. javanicum, ss)为研究对象, 探究了不同生长状态爪哇伪枝藻对高温(45℃)胁迫的响应。结果发现在高温胁迫下, 爪哇伪枝藻光合活性、叶绿素a及类胡萝卜素含量下降; 丙二醛(MDA)、胞外多糖及可溶性蛋白含量上升。在高温处理下, 与fs相比, ss光合活性下降较慢, 且高温处理后ss的叶绿素a及类胡萝卜素含量也明显高于fs。高温处理下, 与fs相比, ss的MDA含量和增长速度均较低; 并且在面临高温胁迫时, ss能够更快的分泌胞外多糖和可溶性蛋白质, 从而在一定程度上达到自我保护的目的。研究结果表明, 在暴露于高温胁迫时, 相较于自由生长状态, 来自地衣的爪哇伪枝藻具有更高的自我保护效率。Abstract: Biological soil crusts (BSCs) are widely distributed in global arid and semi-arid environment, where high temperature stress is one of the critical environmental factors to control the survival of algae. As a common species in BSCs, Scytonema javanicum appears in alga crust stage as free-living form and in lichen crust as symbiotic form to regu-late the formation and development of BSCs. This study explored the effect of high temperature stress (45℃) on symbio-tic (ss) and free-living S. javanicum (fs). The results showed that high temperature stress declined the photosynthetic activity more dramatically in ss compared with in fs, and high temperature stress induced a higher biomass in ss compared with in fs. Compared with fs, ss had lower growth rate and MDA content, and faster extropolysaccharides-released and soluble protein- to protect from damages. The results showed that, compared with free-living form, the S. javanicum from lichens had higher self-protection efficiency when exposed to high temperature.
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Keywords:
- Biological crust /
- Scytonema javanicum /
- Free /
- Symbiosis /
- High temperature stress /
- Physiological and biochemical
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图 1 高温处理对fs和ss的光化学效率的影响
Figure 1. The effect of high temperatures on the maximum photochemical efficiency
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图 2 高温处理对fs和ss的叶绿素a含量的影响
Figure 2. The effect of high temperature on the contents of chlorophyll a
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图 3 高温处理对fs和ss的类胡萝卜素含量的影响
Figure 3. The effect of high temperature on the contents of carotenoid
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图 4 高温处理对fs和ss的丙二醛含量的影响
Figure 4. The effect of high temperatures on the contents of MDA of fs and ss
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图 5 高温处理对fs和ss的胞外多糖含量的影响
不同字母代表差异显著(P < 0.05); 下同
Figure 5. The effect of high temperatures on the contents of EPS of fs and ss
Different letters represent significant differences; the same applies below
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