THE EFFECT OF PGPR ON SUBMERGED MACROPHYTE AND ITS RELATIONSHIP WITH THE SEDIMENT NITROGEN AND PHOSPHORUS FORMS
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摘要: 研究在沉积物高有机质条件下, 通过接种根际促生菌(Plant growth-promoting rhizobacteria, PGPR) PC2(Bacillus stratosphericus)、H19(Bacillus subtilis)和L3(Bacillus cereus)的方式探讨其对苦草(Vallisneria natans)植株的促生效应及其与沉积物氮磷赋存形态的关系。结果表明, 接种组对苦草生长具有显著促进作用, 空白处理种植的苦草生长受到抑制, PGPR对苦草生长促进的综合影响为PC2>H19>L3, PC2处理组株高、根长、地上鲜重和根鲜重比空白分别增加了165.0%、17.4%、378.8%和165.1%。进一步分析不同时期苦草的各种生长指标增量与氮磷赋存形态增量的关系, 通过RDA分析及皮尔森相关分析, 苦草生长指标增量与沉积物中无机氮(Inorg-N)、亚硝态氮(NO2-N)、硝态氮(NO3-N)、无机磷(Inorg-P)和铁/铝磷(Fe/Al-P)等增量显著负相关, 表明PGPR对沉积物中无机态N、P具有一定的控制作用。因此, 接种PGPR对解决受污染湖泊沉水植物恢复及内源污染等问题具有一定潜力。Abstract: As an important primary producer, submerged macrophytes regulate the material circulation and energy flow of the ecosystem, but the recovery process of submerged macrophytes is often plagued by sediments with high organic matter load. The effect of plant growth-promoting rhizobacteria (PGPR) on Vallisneria natans (V. natans) growth and its relationship with sediment nitrogen and phosphorus was studied by inoculating strains PC2 (Bacillus stratosphericus), H19 (Bacillus subtilis) and L3 (Bacillus cereus) under high sediment organic matter load. The results showed that PGPR inoculation significantly promoted the growth of V. natans and the comprehensive effects was PC2>H19>L3. The growth of V. natans grown in the non-inoculated treatment was inhibited. The height, root length, fresh weight of aboveground and underground tissues of V. natans in PC2 treatment group increased by 165.0%, 17.4%, 378.8% and 165.1%, respectively. Through RDA analysis and Pearson correlation analysis, the increment of plant growth index were significantly negatively correlated with the increment of Inorg-N, NO2-N, NO3-N, Inorg-P and Fe/Al-P in the sediment, indicating their potential control sediment Inorg-N and Inorg-P. Therefore, PGPR inoculation is a certain prospective way to solve the problems of submerged macrophytes recovery and internal pollution.
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图 1 实验结束时(120d)四个处理组苦草植株生长情况
Figure 1. Plant growth of V. natans in four treatment groups on the 120th day
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图 2 PGPR接种对苦草的生长变化影响及其显著性分析
字母a—i标识每个生长指标在不同处理组及生长时期的显著性
Figure 2. Effects of PGPR inoculation on the growth of V. natans and their significance analysis
The letters from a to i indicate the significance of each growth index in different treatment groups and time points
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图 6 生长指标增量与沉积物各氮磷形态增量的RDA分析
Figure 6. RDA analysis of the increment between plant growth index and sediment N and P forms
表 1 三株PGPR(PC2、H19、L3)基本信息情况表
Table 1 Basic information of the three strains of PGPR (PC2, H19, L3)
编号Code name 来源Source 菌种Strain 溶磷 Phosphorus solubilisation (mg/L) IAA产生IAA production (mg/L) CKs产生Cytokinins production (μg/L) ACC脱氨酶活性ACC deaminase (U/mg) PC2 菹草根际 Bacillus stratosphericus 35.9±6.6 13.9±0.4 13.3±1.7 0 H19 苦草根际 Bacillus subtilis 11.4±0.2 6.7±0.7 10.1±1.3 0.047±0.006 L3 苦草根际 Bacillus cereus 24.5±0.0 7.3±0.3 15.9±4.7 0.008±0.003 
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表 2 生长指标增量与环境因子增量的相关性分析
Table 2 Correlation analysis between growth index increment and environmental factor increment
Pearson相关指数 ΔTN ΔNH4-N ΔNO3-N ΔNO2-N ΔInorg-N ΔOrg-N ΔTP ΔCa-P ΔFe/Al-P ΔOrg-P ΔInorg-P ∆株高 –0.204 –0.258 –0.835** –0.699** –0.491* –0.201 –0.086 –0.236 –0.391 –0.092 –0.544* Δ根长 0.253 –0.316 –0.544* –0.185 –0.515* 0.168 0.299 0.007 –0.225 –0.015 –0.473* Δ地上鲜重 0.185 –0.184 –0.327 –0.199 –0.209 0.130 0.056 –0.312 –0.565** 0.056 –0.422 Δ根鲜重 0.005 –0.104 –0.249 0.032 –0.126 –0.052 0.285 –0.184 –0.544* 0.115 –0.533* 注: *P,0.05, **P<0.01
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