水环境细菌16S rDNA限制性片段长度多型性及群落结构分析
THE RELATIONSHIP BETWEEN POLYMORPHISM OF BACTERIAL 16S rDNA RESTRICTION FRAGMENT LENGTH AND COMMUNITY STRUCTURE OF AQUATIC BACTERIA
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摘要: 用细菌16S核糖体RNA基因(rDNA)限制性片段长度多型性描述了水环境细菌群落结构.从环境水样中直接分离 DNA,以细菌特异的引物扩增 165 rDNA,构建质粒文库,随机分离重组质粒,用限制性内切酶消化获得 16S rDNA基因型,用基因型的种类及频率描述特定水体生境的细菌群落结构.该方法在分析水体隐含遗传多样性、揭示污染的生物学效应和评价水环境质量等方面具有重要的应用价值.
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关键词:
- 16S核糖体RNA基因 /
- 限制性片段长度多态性 /
- 细菌群落 /
- 水环境
Abstract: In this study, a technique describing aquatic bacterial community structure has been developed by using restriction fragment length polymorphism of 16S ribosomal RNA gene(rDNA). The technique includes isolation of DNA directly from lake-water, amplification of rDNA using bacteria-specific primers, construction of plasmid library and digestion of randomly extracted recombinant plasmids with restriction endonuclease. The structure of bacterial community is determined using rDNA genotypes and their frequencies. This method has potentials in analyzing hidden genetic diversity of aquatic environments, tracing biological effectiveness of pollutants and evaluating environmental quality. -
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