摘要:
采用气相色谱、组织学及电镜技术分别对中华绒螯蟹雄蟹白化症肝胰腺与正常肝胰腺脂类和脂肪酸组成、显微及超微结构进行了比较研究,结果表明:正常肝胰腺的总脂含量(31.54%肝胰腺湿重)显著高于白化症肝胰腺(19.07%肝胰腺湿重),两种蟹肝胰腺的脂类组成没有显著差异,但脂肪酸组成存在一定的差异,白化症肝胰腺中C18∶1的含量显著高于正常肝胰腺,中性脂和极性脂HUFA正常蟹肝胰腺含量都高于白化症蟹,但没有显著差异。组织学结果和超微结构观察表明:白化症肝胰腺肝小管的上皮细胞壁结构紊乱,微绒毛及微绒毛黏膜大部分脱落,细胞内仅见有零星的线粒体、脂肪滴分布,R细胞呈现空泡化。
关键词:
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中华绒螯蟹
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肝胰腺
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脂肪酸
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结构
Abstract:
In recent years, Eriocheir sinensis with white hepatopancreas was frequently observed in the cultivation p rocess,and the white hepatopancreas was called hepatopancreas albinism, but the albino hepatopancreas was mainly observed inmale crabs.The study was investigated to determine the factors and causes resulting in that phenomenon during the Eriocheir sinensis cultivation process.The animals used in this experiment were collected from the different cultivation locations in the YangchengLake.Theywere quickly sacrificed after being taken to the laboratory, and then hepatopancreaswere dissected from the experimental crabs.The lipid classes, fatty acid composition and microultrastructure of the crabswith albino hepatopancreaswere determined, compared with those crabswith normal hepatopancreas.According to the results, significant difference wasfound at hepatosomatic index and total content of lipid.The lipid classes of two kinds crabswere almost the same, the contents of neutral lipid were 86.45% in normal hepatopancreas and 89.30% in albino, and the main componentwas triacylg lyceride.The content of C.8:. was 8.00% and 37.75% , respectively, higher than any other kind of fatty acid in neutrallip id.The contents of C5∶0, C6∶., C0∶. and EPA of normal crabs were higher than albino crabs, but the contents ofC.6∶0 and C.8∶. were opposite.The fatty acid composition of polar lip id in the hepatopancreas of two kinds of crabswassimilar to that of the neutral lip id.The content of C.8∶. was the highest and the content of albino crabs was significanthigher than normal ones (p<0.0.).In normal crabs, epithelial cell wall of hepatic tubule was comp rised of monolayercells.B cell and R cell arranged in the order.The microvilli and enteric coat at the top of cells showed comp lete structure.The lumen of hepatic tubule contained some inclusion. In contrast, the cells of ep ithelial cellwall of hepatic tubule arranged in disorder in the albino crabs.The microvilli and its mucosal disappeared. Cavity appeared irregularly in hepatictubule, and cells separating from lumen walls were observed. It is estimated that the color of hepatopancreas has tight connection with differences of lipid content.The hepatopancreasof normal crabs have low content of water but high content of lipid, therefore, it contained more lipidsoluble vitamins andcoloringmatters than albino ones,which leads to yellow color.However, the result of albinol oneswas opposite.The accumulation of lipid soluble vitamins and coloringmatterswere prevented,which leads to white color.According to some surveys, fattyacid composition and microultrastructure of albino hepatopancreas cells has tight relationship to the lack of EFA.
百度学术
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