富硒螺旋藻培养技术研究
A STUDY OF THE SE-RICH CULTIVATION TECHNOLOGY OF SPIRULINA PLATENSIS
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摘要: 采用富硒技术对钝顶螺旋藻培养进行强化,对硒(IV)浓度和亚硫酸盐的影响,以及硒的生物富集及其对藻细胞分子官能团结构的影响等进行了较为详细的研究,并对相关的可能机理进行了讨论.研究发现,硒对钝顶螺旋藻生长具有刺激或抑制的双重作用.在0.02mg/L～411.00mg/L浓度范围内,硒不仅可以加快钝顶螺旋藻的生长,而且还可以提高其生物量;同时,钝顶螺旋藻对硒的生物富集随着硒浓度的增加而增加,较为缓慢的生长利于钝顶螺旋藻对硒的富集.研究还证实,Na2SO3会减轻高浓度Na2SeO3对钝顶螺旋藻生长的毒性,富硒培养不会对藻细胞分子官能团结构产生损害.实验得出钝顶螺旋藻富硒培养较佳的硒处理浓度在10mg/L～40mg/L.Abstract: In this paper, the cultivation of Spirulina Platensis was enhanced by Na2SeO3 addition in the medium. Some factors such as Se(IV)concentration, sulphite and Se bioaccumulation as well as its effect on the algal cell molecular functional group structure etc. were investigated in detail. In addition, the relevant mechanisms were discussed. It was found that Se(IV)had both stimulation inhibition effects on the culture of Spirulina platensis. Within the concentration range of 0.02mg/L—411.00mg/L, Se(IV)not only can stimulate the growth speed of Spirulina platensis, but also can improve its biomass yield. Se bioaccumulation in Spirulina platensis will rise up with the increase of Se(IV)concentration and will beneffit from the algal slow growth. The toxicity of higher concentration selenite on the growth of Spirulina platensis could be decreased by sulphite addition. There is no damage to the molecular functional group structure of Spirulina platensis during Se-enrichment culture. On the basis of this study, the appropriate Se(IV)concentration range for Spirulina platensis cultivation is about 10mg/L—40mg/L.
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Keywords:
- Spirulina platensis /
- Se /
- Enhanced cultivation /
- Bioaccumulation
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[1] 杨逸萍.硒在海洋浮游植物生化组分中的分布[J].海洋学报,1994,(3);143—148[2] Zhang G H Se Uptake and Accumulation in Marine Phytoplankton and Tranfer of Se to the Clam Puditapes Philippnarum[J].Marine Environ.Res.,1990,30:179—190[3] James R Pratt.Effect of Se on Microbial Communities in Laboratory Microcosms and Outdoor Streams[J].Toxic.Assess.1990,5:293—307[4] 徐辉碧,范华汉.硒.生命科学中的微量元素(第二版)[M].北京;中国计量出版社.1996;620—668[5] 李志勇,郭祀远,李琳,等.富含微量元素的功能性食品[J],食品科技,1997,(3);12—14[6] 李志勇,郭祀远,李琳,等.功能性螺旋藻保健食品的研制与开发[J].粮食与饲料工业,1997,(10);37—39[7] Rejean Samson.Multistage Continuous Cultivation of Blue-green Alga Spirulina maxima in the Flat Tank Photobioreactors with Recyde[J].Can.J.Chem.Eng.,1985,63:105—112[8] 李志勇,郭祀远,李琳,等.鼓泡柱式光生物反应器培养螺旋藻的研究[J].食品工业科技,1998,(5);18—21[9] 周志刚.硒对极大螺旋藻生长及含硒量的影响[J].海洋科学,1997,(5);42—45[10] 李志勇,郭祀远.高附加值微藻的高细胞密度培养[J].海湖盐与化工.1999,(3);7—10[11] 李志勇,郭祀远,李琳,等.螺旋藻的大规模工业化生产[J].海湖盐与化工.1998,(1);38—44[12] 李志勇,郭祀远,李琳,等.藻类对微量元素的生物富集及其应用[J].微生物学通报.1997,(6);368—369 杨逸萍.硒在海洋浮游植物生化组分中的分布[J].海洋学报,1994,(3);143—148[2] Zhang G H Se Uptake and Accumulation in Marine Phytoplankton and Tranfer of Se to the Clam Puditapes Philippnarum[J].Marine Environ.Res.,1990,30:179—190[3] James R Pratt.Effect of Se on Microbial Communities in Laboratory Microcosms and Outdoor Streams[J].Toxic.Assess.1990,5:293—307[4] 徐辉碧,范华汉.硒.生命科学中的微量元素(第二版)[M].北京;中国计量出版社.1996;620—668[5] 李志勇,郭祀远,李琳,等.富含微量元素的功能性食品[J],食品科技,1997,(3);12—14[6] 李志勇,郭祀远,李琳,等.功能性螺旋藻保健食品的研制与开发[J].粮食与饲料工业,1997,(10);37—39[7] Rejean Samson.Multistage Continuous Cultivation of Blue-green Alga Spirulina maxima in the Flat Tank Photobioreactors with Recyde[J].Can.J.Chem.Eng.,1985,63:105—112[8] 李志勇,郭祀远,李琳,等.鼓泡柱式光生物反应器培养螺旋藻的研究[J].食品工业科技,1998,(5);18—21[9] 周志刚.硒对极大螺旋藻生长及含硒量的影响[J].海洋科学,1997,(5);42—45[10] 李志勇,郭祀远.高附加值微藻的高细胞密度培养[J].海湖盐与化工.1999,(3);7—10[11] 李志勇,郭祀远,李琳,等.螺旋藻的大规模工业化生产[J].海湖盐与化工.1998,(1);38—44[12] 李志勇,郭祀远,李琳,等.藻类对微量元素的生物富集及其应用[J].微生物学通报.1997,(6);368—369
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