斑点叉尾鮰套肠症的病原学初步研究
PRELIMINARY STUDY ON THE ETIOLOGY OF CHANNEL CATFISH INTUSSU SCEPTION DISEASE
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摘要: 本研究从2例垂死的患败血症并肠套叠的斑点叉尾鮰血液、肝脏及脑组织中分离到2种类型的致病性细菌菌株,经对细菌进行形态观察、生理生化测试和16S rDNA序列分析,分别鉴定为嗜水气单胞菌和荧光假单胞菌;攻毒实验证实它们确为斑点叉尾鮰病鱼的病原。根据药敏实验的结果,及时对病区斑点叉尾鮰进行治疗,连续用药3-5d,发病的叉尾鮰即停止死亡。根据研究结果,文章对斑点叉尾鮰肠套叠发生的原因进行了的分析,提出了不同的观点。Abstract: Nowadays,a named "intussusception" disease in channel catfishes occurred in many latitudes of China.Besides haemorrhage symptom,the disease exhibit intestine shrinking,intussusception,rectocele phenomenon and so on.The disease mostly happened in spring and summer,sometimes in October,and it have resulted in so many channel catfish dead in China for years that menace the healthy culturing strictly.However,this disease has been analysed and studied by some domestic and international scholars,the undoubted nosogenesis has not been found now.Some scholars considered that intussusception disease was developed by Stenotrophomonas maltophilia,others deemed that it maybe not an independent one,only a familiar symptom in channel catfish.So far,formal coverage about other pathogenic bacteria in channel catfish has not been turned up.So,the etiology of channel catfish intussusception disease was studied preliminarily in this study through two kinds of diseased channel catfish suffered intussusception in China.In this study,two kinds of pathogenic strains named YDCC-5-1,0704CCBr and 0704CCl were isolated from the blood respectively,liver and brain of moribund channel catfish which suffered intussusception with integrated intestine collected from two different regions in Hubei Province.The two diseased fishes showed the similar symptom of haemorrhage:eyes,operculum,pars cephalica,the base of pectoral fin and pelvic fin hyperaemia,anus hyperaemia,and some suffered typical intussusception.The concentration of dissolved oxygen,union-ammonia and nitrite in the water areas of two diseases were all in margin of safety.The isolate YDCC-5-1 was identified as Aeromonas hydrophila controlled by Aeromonas hydrophila(XS91-4-1),and the isolates 0704CCBr and 0704CCl were identified as Pseudomonas fluoresces controlled by Pseudomonas fluoresces(56-12-10),respectively,through morphological observation,physiological and biochemical tests and analysis of 16S rDNA sequence.The strains XS91-4-1 and 56-12-10 were isolated and preserved by this laboratory,used as control strains in physiological and biochemical tests.The isolate YDCC-5-1 was identical with A.hydrophila in morphological observation,physiological and biochemical tests and analysis of 16S rDNA sequence:round colony,single thalli or bigeminal,bacilliform and Gram reaction negative,positive for motility,oxidatase,catalase,M.R and V-P reaction,fermented glucose,arabinose,sucrose and so on;16S rDNA sequence of YDCC-5-1 was 99% similar to A.hydrophila ATCC7966 in GenBank.The isolates 0704CCBr and 0704CCl grow at 4℃,not at 41℃,give birth to fluorescence pigment,oxidatase and catalase,fermented glucose,utilize glucose,fucose,sucrose,L-proline only as carbon source,Gram reaction negative,which were all identical with Pseudomonas fluoresces combined with analysis of 16S rDNA sequence.In addition,they were all proved to be pathogenic to experimental fish in the challenge testes.The two cases of channel catfish intussusception diseases were controlled by using antibiotics based on the drug sensitivity of these isolates.Thereinto,florfenicol was recommended to breed aquatics in Yidu Qingjiang,Hubei Province,and norfloxacin was commended to breed aquatics in Niushan Lake,Wuhan,Hubei Province,according as the result of the drug sensitivity of these isolates.According to our study results,new viewpoint and speculation of channel catfish intussusception disease was discussed,however,the further study should be continued rapidly.
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Keywords:
- Ictalurus punctatus /
- Aeromonas hydrophil /
- Pseudomonas fluoresces /
- Intussusception
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