白鲢个体发育过程中同工酶基因的表达与调控研究
STUDIES ON THE EXPRESSIONS AND REGULATIONS OF ISOZYMIC GENES IN SILVER CARP (HYPOPHTHALMICHTHYS MOLITRIX)DURING ONTOGENESIS
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摘要: 采用淀粉或聚丙烯酸胺凝胶电泳及特异性组织化学染色技术研究了白鲢早期发育过程中(从未受精卵到卵黄吸尽期)及成体不同组织(脑、眼、心、肌、肾、肝)中六种同工酶系统(LDH,MDHIDHADHSDH,EST)的分化表达谱式。白鲢各同工酶基因的表达具有明显的组织特异性。早期发育过程中,ADH和SDH基因均无活性,其它四种同工酶则具有不同的个体发育谱式。值得一提的是,由取自同一地区的两对白鲢的受精卵的形成胚胎的LDH和EST同工酶的个体发育谱式具有明显的差异。这种差异很可能与不同白鲢母本的未受精卵中不同的同工酶组成有关。本文最后还提出了今后深入研究的有关问题。Abstract: The patterns of expressons and regulations in six isozymic systems (LDH, MDH, IDH,ADH, SDH and EST) were investigated in the early deveopmental stages (from unfertilized eggs to the stage of complcte yolk absoption) as well as six differentiated adult tissues (brain, eye, heart, muscle, kidney and liver) of silver carp. Isozymes were resolved by starch or polyacrylamide gel elctrophoresis and then detected by specific histochemical staining. Isozymes in adult silver carp apparently exhibit a tissue-specific expression.During the early developmenta period, both ADH and SDH genes seem to be inactive;the other four isozyme genes are active but exhibit different patterns of expressions.It is noteworthy, however that both LDH and EST genes in embryo from two pairs of silver carp parents taken from the same locality exhibit different patterns of expressions and regulations.We argued that there differences are related to the different complsitions of LDH and EST isozymes in unfertilized eggs of different female parents.Some related problems which ought to be further studied are also mentioned in the present paper.
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