固定化微绿球藻去除NH4+-N、PO43--P效果的研究
STUDY ON REMOVAL RATE OF NH4+-N AND PO43--P BY IMMOBILIZED NANNOCHLOROPSIS OCULATA
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摘要: 为了探究固定化微绿球藻（Nannochloropsis oculata）去除污水中NH4+-N、PO43--P的效果，采用海藻酸钠固定化包埋技术进行实验。开展了固定化藻球大小、藻细胞包埋密度、藻球投放质量及充气培养条件对NH4+-N、PO43--P去除效果的单因子试验研究。结果表明，固定化藻球大小、藻细胞包埋密度、藻球投放质量和充气培养条件对NH4+-N、PO43--P的去除效果影响显著（P0.05）。藻球直径3.5 mm时生长速率（K）值最大（0.3320.002），同时NH4+-N、PO43--P去除率效果最佳，分别为（75.083.83）%和（80.803.81）%；藻细胞包埋密度100104 cells/ball时K值最大（0.3300.033），而NH4+-N、PO43--P去除率则以藻细胞包埋密度300104 cells/ball组为佳，分别达（87.200.43）%和（82.581.72）%，但考虑单位藻细胞去除率，包埋密度以100104 cells/ball为宜；随着藻球用量的增加K值下降，10 g/L组K值最大（0.3010.02）、50 g/L组K值最小（0.1930.01），投放量30和50 g/L时NH4+-N去除率较高分别为（84.120.78）%和（84.630.45）%，30 g/L组PO43--P去除率最高达（77.131.43）%。综合考虑，藻球投放量选用30 g/L为宜；充气条件培养K值、NH4+-N和PO43--P去除率显著（P0.05）高于不充气，K值分别为（0.3060.006）和（0.1770.010）；NH4+-N去除率分别为（85.930.45）%和（49.320.45）%；PO43--P去除率分别为（66.665.00）%和（46.292.12）%。研究优化了微绿球藻固定化条件：固定化微绿球藻应进行充气培养，藻球规格3.5 mm、藻细胞包埋密度100104 cells/ball、藻球投放量30 g/L。Abstract: Microalgae Nannochloropsis oculata, immobilized with sodium alginate, was used to explore its removal efficiency of NH4+-N and PO43--P from artificial sewage water. Algae ball size, cell densities, dosages of algae balls, and aeration cultured were applied in the single-factor. The results showed that all these conditions significant impact the removal of of NH4+-N and PO43--P. The growth rate of K value achieved the highest value (0.3320.002) when the diameter was 3.5 mm; the removal rate of NH4+-N and PO43--P were the highest one at the diameter was 3.5 mm, which were (75.083.83)% and (80.803.81)%, respectively. The maximum of the growth K values was (0.3300.033) with the density of 100104 cells/ball. The highest NH4+-N and PO43--P removal rate were (87.200.43)% and (82.581.72)%, respectively, at the group of 300104 cells/ball; however, 100104 cells/ball was the optimal algal cell density based on unit algal cells removal ratio of NH4+-N and PO43--P. The increased algae balls dosages decreased the growth rate of K values. 10 g/L group had the maximum K values (0.3010.02) and 50 g/L had the minimum K values (0.1930.01). The removal rates of NH4+-N were (84.120.78)% and (84.630.45)% when the dosage was 30 g/L and 50 g/L, respectively. 30 g/L group had the highest PO43--P removal rate (77.131.43)%. Combined analyses revealed that 30 g/L was optimum for the dosages of algae balls. The K value, the removal of NH4+-N and PO43--P were significantly (P0.05) higher with aeration than non-aerated; K values were (0.3060.006) and (0.1770.010), respectively; NH4+-N removal rates were (85.930.45)% and (49.320.45)%, respectively; PO43--P removal rates were (66.665.00)% and (46.292.12)%, respectively. This study optimized the conditions of immobilized microalgae Nannochloropsis oculata:immobilized Nannochloropsis oculata should be aerated cultures; algae ball size was 3.5 mm; the algal cell density was 100104 cells/ball; and the dosage of algae balls was 30 g/L.
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Keywords:
- Nannochloropsis oculata /
- Immobilization /
- NH4+-N /
- PO43--P /
- Removal rate
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