八肋游仆虫γ-微管蛋白及其基因的研究

基金项目:

国家自然科学基金

STUDIES ON THE γ-TUBULIN AND GENE IN EUPLOTES OCTOCARINATUS

摘要: γ－微管蛋白是一种新发现的与微管形成有关的蛋白质[1]。利用PCR技术从一种下毛类纤毛虫─—八肋游仆虫大核DNA中扩增出γ－微管蛋白基因并对其核昔酸序列进行了分析。用抗γ－微管蛋白的多克隆抗体标记八肋游仆虫发现:γ－微管蛋白存在于八肋游仆虫的纤毛、棘毛的基部─—即基体的部位。另外,在有些分裂间期及有丝分裂期的小核内以及部分大核内也发现有γ－微管蛋白的存在。
- 纤毛虫 /
- 微管蛋白 /
- 聚合酶链反应
Abstract: γ-tubulin is a newly discovered protein, which is considered to be essentialfor microtubule nucleation.With the help of the polymerase chain reaction(PCR) weamplified and sequenced the γ-tubulin gene of Euplotes octocarinatus, a hypotrichousciliate.Polyclonal antibody to localize mis protein in E.octocarinatus and found, thatγ-tubulin is present in the basal bodie of dordal cilia,the basal bodies of cirri and the basal bodies of membranelles.In addition,it was found in the micronucleus and andin the macronucleus.
- Ciliate /
- Tubulin /
- PCR