离心力对制备沉积物间隙水中化合物浓度的影响
EFFECT OF CENTRIFUGAL FORCE ON COMPOUNDS CONCENTRATIONS IN SEDIMENT INTERSTITIAL WATER
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摘要: 研究了离心力对间隙水化合物浓度的影响.将腐殖质掺入东湖沉积物样品,按泥:水=1:4(体积)比室温下静置30d,随后掺入铜,泥:水=1:4(体积)比静置10d,得到总有机碳含量在1.47%-.72%、铜含量在120-2700mg/kg(干重)的试验沉积物样品,用离心法制备沉积物中的间隙水.离心参数为3000r/min、200r/min、9000r/min、1000r/min和12000r/min,4 ℃下离心20min.间隙水中化合物浓度分析表明,随离心力升高,间隙水中铜、铁、总有机碳(TOC)含量逐渐降低,钙的含量略有增加,而锰的含量不受离心力变化的影响.根据上述分析结果,确定在腐殖质对沉积物中铜的毒性影响研究中,制备沉积物间隙水的适宜离心条件为:12000r/min、4℃下离心20min.Abstract: Freshwater sediment from Eastlake, Wuhan, China, was spiked with humus and copper to obtain a series of different levels of total organic carbon (TOC, 1.47%—5.72%)and total copper (120—2700mg/kg dry weight). In laboratory, humus was added to natural freshwater sediment and stored at 20℃ for 30 days in a volume portion of 1:4 (sediment to water), then samples was spiked with copper and stored for 10 days. These humus and copper-spiked sediments were centrifuged under 3000r/min, 5200r/min, 9000r/min, 10500r/min, 12000r/min, 4℃ and 20min to collected its interstitial water (IW). chemica1 analysis showed that, Cu, Fe, TOC concentrations in IW decreased as centrifugal speed increased, meanwhile Ca somewhat increased but Mn concentration had no apparent correlation with centrifugal speed. According to the above results, the centrifugation method for interstitial water was preferable at: 12000r/min(9000g RCF), 4℃, 20min in study of humus effect on the toxicity of copper in sediment.
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Keywords:
- Sediment /
- Interstitial water(IW) /
- Centrifugal speed /
- Concentration
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Toxicol., 1994,27:507-519[7] Poston T J, Purdy R,Aquatic Toxicology and Environmental Fate ASTM STP 921 [M], Philadelphia, American Society for Testing and Materials, 1986,479-493[8] Swartz R C, Kemp P F, Schults D W, et al. Acute toxicity of sediment from Eagle Harbor, Washington, to the infaunal Amphipod Rhepoxynius Abronius.[J]Environ Toxicol. Chem., 1989,8:215-222[9] Ankley G T, Katko A, Arthur J. Identificatiou of ammonia as an important sediment -associated toxicant in the lower Fox River and Green Bay, Wisconsin[J]. Environ. Toxicol. Chem., 1990,9:313-322[10] Sarda N, Burton G A. Ammonia variation in sediments: spatial, temporal and method-related effected [J]. Environ. Toxicol. Chem., 1995,14:1499-1506[11] Frazier B E, Naimo T J, Sandheinrich M B. Temporal and vertical distribution of tota1 ammonia nitrogen and un-ionized ammonia nitrogen in sediment pore water from the upper Mississippi River [J]. Environ. Toxciol. Chem., 1996,15:92-99[12] Burton C S. Developing of formulated reference sediments for freshwater and estuarine sediment testing [J]. Environ. Toxicol. Chem., 1994, 13:1163-1175[13] Adams D D, Darby D A, Young R J. Selected analytical techniques for characterizing the metal chemistry and geology of fine-grained sediments and interstitial water [A]. Robere A. Contaminants and sediment [C]. Arbor:Ann Arbor Sciences publishers. 1980
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