嗜水气单胞菌和河弧菌二联疫苗对鲫的免疫效果
DEVELOPMENT AND IMMUNE EFFICACY OF A BIVALENT ANTI-AEROMONAS HYDROPHILA AND ANTI-VIBRIO FLUVIALIS VACCINE IN CARASSIUS AURATUS LINNAEUS
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摘要: 在采用正交试验设计优化了Aeromonas hydrophila和Vibrio fluvialis二联全菌疫苗制备工艺的基础上,研究了采用该工艺所制备的疫苗对鲫的免疫保护效果。结果表明注射免疫鲫4周后,抗A. hydrophila和V. fluvialis血清凝集效价分别为:1∶4—1∶32和1∶2—1∶16,平均都为1∶10,但对攻毒均具有有效的保护作用。浸泡免疫、高渗浸泡免疫或加多糖浸泡免疫在免疫4周后,对A. hydrophila攻毒均无保护作用;然而,加多糖浸泡免疫对V. fluvialis攻毒可产生有效的保护。免疫扩散试验表明,在煮沸并经超声波处理后的A. hydrophila或V. fluvialis菌液中均存在两种优势抗原。Abstract: Aeromonas hydrophila and Vibrio fluvialis are the causative agents pathogenic bacterial of haemorrhagic septicemia of a variety of farmed fish in China. Abivalent vaccine against these two pathogens was being developed by an orthogonal array design. The optimized bivalent vaccine could elicit effective protection from experimental challenge of A. hydrophila for C. auratus 4 weeks after immunized by i.p injection, but not by neither immersion vaccination nor hyperosmotic infiltration with or without supplement of polysaccharide. The vaccine was also protective against the challenge of V. fluvialis when fish was immunized by i.p injection and immersion vaccination plus polysaccharide. At challenge time, serum agglutination titers from fish injected with the bivalent vaccine were 1∶4-1∶32, 1∶2-1∶16, results of immunodiffusion indicated that there were two dominant antigens in boiled and sonicated V. fluvialis and A. hydrophila vaccine.
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Keywords:
- Bivalent vaccine /
- Aeromonas hydrophila /
- Vibrio fluvialis /
- Carassius auratus
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[1] Hernanz MC, Flano del CE, Lopez, et al. Molecular characterization of the Aeromonas hydophila aroA and potential use of an auxotrophic aroA mutant as a live attenuated vaccine [J]. Infect Immun, 1998, 66(5):1813-1821[2] Lutwycge P, Exner MM, Habcocck RE, et al. A conserved Aeromonas salmonicida porin provides protective immunity to rainbow trout [J]. Infect Immun, 1995, 63:3137-3142[3] Dooiev JGS, Lallier R, Trust TJ. Surface antigens of virulent strains of Aeromonas hydrophila [J]. Vet Immunol Immunopathol, 1986, 12:339-344[4] 徐伯亥,殷战,吴玉深,等.淡水养殖鱼类爆发性传染病致病细菌的研究[J]. 水生生物学报,1993,17:259-265[5] Lorenzen N, Lorenzen E, et al. Protective immunity to VHS in rainbow trout following DNA vaccination [J]. Fish shellfish immunol, 1988, 8:261-270[6] Leblanc D, Mittal KR, Olivier G, et al. Serogrouping of motile Aeromonas hydrophila isolated from healthy and moribund fish [J]. Appl and Environ Micro, 1981, 42(1): 56-60[7] Post G. Response of rainbow trout to antigens of Aeromonas hydrophila [J]. J Fish Res Bd Can, 1966, 23:1487-1494[8] Schacte JH. Studies on the immunization of the channel catfish against two bacterial pathogens [D]. Ph.D. Thesis, Auburn University, Auburn AL, 1-89[9] Lamers CH De Haas MJ. Antigen localization in the lymphoid organs of carp (Cyrinus carpio)[J]. Cell Tissue Res, 1985, 242A(3):491-498[10] Ruangapan L, Kitao T, Yoshida T. Protective efficacy of Aeromonas hydrophila vaccines in Nile tilapia [J]. Vet. Immunol Immunopathol, 1986, 12:345-350[11] Baba T, Imamura J, Izawa K, Ikeda K. Cell-mediated protection in carp against Aeromonas hydrophila [J]. J Fish Dis, 1988, 11:171-178[12] Kurunasagar I, Rosalind G. Immunological response of the Indian major carps to Aeromonas hydrophila vaccine [J]. J Fish Dis, 1991,14:413-417[13] 陈月英,钱冬,沈智华,等.淡水鱼类细菌性败血症菌苗浸泡免疫研究[J].海洋与湖沼,1998,29(6):597-603 Hernanz MC, Flano del CE, Lopez, et al. Molecular characterization of the Aeromonas hydophila aroA and potential use of an auxotrophic aroA mutant as a live attenuated vaccine [J]. Infect Immun, 1998, 66(5):1813-1821[2] Lutwycge P, Exner MM, Habcocck RE, et al. A conserved Aeromonas salmonicida porin provides protective immunity to rainbow trout [J]. Infect Immun, 1995, 63:3137-3142[3] Dooiev JGS, Lallier R, Trust TJ. Surface antigens of virulent strains of Aeromonas hydrophila [J]. Vet Immunol Immunopathol, 1986, 12:339-344[4] 徐伯亥,殷战,吴玉深,等.淡水养殖鱼类爆发性传染病致病细菌的研究[J]. 水生生物学报,1993,17:259-265[5] Lorenzen N, Lorenzen E, et al. Protective immunity to VHS in rainbow trout following DNA vaccination [J]. Fish shellfish immunol, 1988, 8:261-270[6] Leblanc D, Mittal KR, Olivier G, et al. Serogrouping of motile Aeromonas hydrophila isolated from healthy and moribund fish [J]. Appl and Environ Micro, 1981, 42(1): 56-60[7] Post G. Response of rainbow trout to antigens of Aeromonas hydrophila [J]. J Fish Res Bd Can, 1966, 23:1487-1494[8] Schacte JH. Studies on the immunization of the channel catfish against two bacterial pathogens [D]. Ph.D. Thesis, Auburn University, Auburn AL, 1-89[9] Lamers CH De Haas MJ. Antigen localization in the lymphoid organs of carp (Cyrinus carpio)[J]. Cell Tissue Res, 1985, 242A(3):491-498[10] Ruangapan L, Kitao T, Yoshida T. Protective efficacy of Aeromonas hydrophila vaccines in Nile tilapia [J]. Vet. Immunol Immunopathol, 1986, 12:345-350[11] Baba T, Imamura J, Izawa K, Ikeda K. Cell-mediated protection in carp against Aeromonas hydrophila [J]. J Fish Dis, 1988, 11:171-178[12] Kurunasagar I, Rosalind G. Immunological response of the Indian major carps to Aeromonas hydrophila vaccine [J]. J Fish Dis, 1991,14:413-417[13] 陈月英,钱冬,沈智华,等.淡水鱼类细菌性败血症菌苗浸泡免疫研究[J].海洋与湖沼,1998,29(6):597-603
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